Molecular Cloning of a Putative Novel Human bZIP Transcription Factor on Chromosome 17q22

• Published in: Genomics (San Diego, Calif.) Vol. 22; no. 3; pp. 553 - 562
• Main Authors: Luna, Luisa, Johnsen, Øyvind, Skartlien, Anne Helen, Pedeutour, Florence, Turc-Carel, Claude, Prydz, Hans, Kolstø, Anne-brit
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1994; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Basic-Leucine Zipper Transcription Factors; Biological and medical sciences; Caenorhabditis elegans - genetics; Chromosome Mapping; Chromosomes, Human, Pair 17; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Consensus Sequence; DNA, Complementary - genetics; DNA-Binding Proteins - genetics; Drosophila melanogaster - genetics; Fundamental and applied biological sciences. Psychology; G-Box Binding Factors; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; In Situ Hybridization, Fluorescence; Leucine Zippers - genetics; Molecular Sequence Data; RNA, Messenger - genetics; Sequence Homology, Amino Acid; Transcription Factors - genetics; Genetic mapping; Human; E17-Chromosome; Alternative splicing; Leucine zipper structure; Complementary DNA; Nucleotide sequence; Tissue specificity; Homology; Gene organization; Gene expression; Transcription factor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1994.1428

We have cloned and characterized cDNA clones representing several mRNA isoforms generated by alternative splicing of a single gene localized to chromosome some 17q22. Sequence analysis showed that the predicted translational product of the longest open reading frame (2316 nucleotides, 772 amino acids) is related to transcription factors of the basic leucine zipper (bZIP) class. The sequence contained several regions characteristic of transcriptional regulatory domains. A cluster of amino acids flanking the bZIP region on both sides was highly conserved between TCF11 and p45 NF-E2, a subunit of the human globin locus control region-binding protein, NF-E2. These same regions showed remarkable homology to two invertebrate proteins, CNC and skn-1, postulated to regulate embryonic development in Drosophila melanogaster and Caenorhabditis elegans, respectively.