Functionalization of PVDF membranes with carbohydrate derivates for the controlled delivery of chlorhexidin

• Published in: Biomolecular engineering Vol. 24; no. 5; pp. 472 - 476
• Main Authors: Tabary, N., Lepretre, S., Boschin, F., Blanchemain, N., Neut, C., Delcourt-Debruyne, E., Martel, B., Morcellet, M., Hildebrand, H.F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2007
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacokinetics; Anti-Bacterial Agents - pharmacology; Carbohydrates - chemistry; Cell Line; Cell Proliferation - drug effects; Cell Survival - drug effects; Chlorhexidine; Chlorhexidine - analogs & derivatives; Chlorhexidine - chemistry; Chlorhexidine - pharmacokinetics; Chlorhexidine - pharmacology; Citric Acid - chemistry; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Controlled release; Cyclodextrin; Cytocompatibility; Drug Delivery Systems; Fusobacterium nucleatum - drug effects; Guided Tissue Regeneration; Humans; Maltodextrin; Membranes, Artificial; Microbial Sensitivity Tests; Microbiology; Polysaccharides - chemistry; Polyvinyls - chemistry; Porosity; PVDF membrane; Surface Properties; Cyclodextrin; Cytocompatibility; Microbiology; Maltodextrin; PVDF membrane; Chlorhexidine; Controlled release; Guided Tissue Regeneration
• ISSN: 1389-0344; 1878-559X
• DOI: 10.1016/j.bioeng.2007.07.007

Maltodextrin (MX) was fixed onto PVDF membranes in order to create a drug delivery Guided Tissue Regeneration (GTR) device with controlled drug delivery properties. PVDF microporous membranes were treated by a mixture of MX and citric acid, resulting to an 18 wt% increase of the supports. MX grafted membrane could capture 103 mg/g chlorhexidin digluconate (DigCHX) instead of 1 mg/g for a virgin membrane. A neutralization step was performed before the biological tests. Viability tests confirmed the non-toxicity of the MX polymer coating after neutralisation. In vitro release test in human plasma, and microbiological tests showed that membranes grafted with MX were more performing compared to virgin and β-CD grafted membranes. The antimicrobial activity was effective during more than 72 h.