Analysis of metabolic changes in Trichoderma asperellum TJ01 at different fermentation time-points by LC-QQQ-MS

Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to primary and secondary metabolites. This study investigated the different metabolites in Trichoderma asperellum TJ01 cultured for 24 and 72 h using...

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Published inJournal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 54; no. 1; pp. 20 - 26
Main Authors Ni, Mi, Wu, Qiong, wang, Gui S., Liu, Qian Q., Yu, Mei X., Tang, Jun
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 02.01.2019
Taylor & Francis Ltd
Subjects
Alkaloids
Amino acids
Biological control
Culture
different time-points
Fermentation
Flavonoids
Fungi
Hormones
Lipid metabolism
Lipids
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Plant growth
Polyphenols
Quadrupoles
Secondary metabolites
Sugar
Trichoderma asperellum
triple-quadrupole mass spectrometry
different time-points
fermentation
metabolites
triple-quadrupole mass spectrometry
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Abstract Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to primary and secondary metabolites. This study investigated the different metabolites in Trichoderma asperellum TJ01 cultured for 24 and 72 h using liquid chromatography with triple-quadrupole mass spectrometry. Compared to the 24 h culture of T. asperellum TJ01, the 72 h culture with amino acid metabolism tended to decrease while sugar and lipid metabolisms tended to increase. Furthermore, the 72 h culture had a higher proportion of upregulated flavonoids, in combination with a higher proportion of downregulated alkaloids, and equal proportions of upregulated and downregulated polyphenols and hormones. This study also identified a few valuable medicinal substances such as trigonelline and 5-hydroxytryptophan in T. asperellum TJ01 fermentation cultures.
AbstractList Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to primary and secondary metabolites. This study investigated the different metabolites in Trichoderma asperellum TJ01 cultured for 24 and 72 h using liquid chromatography with triple-quadrupole mass spectrometry. Compared to the 24 h culture of T. asperellum TJ01, the 72 h culture with amino acid metabolism tended to decrease while sugar and lipid metabolisms tended to increase. Furthermore, the 72 h culture had a higher proportion of upregulated flavonoids, in combination with a higher proportion of downregulated alkaloids, and equal proportions of upregulated and downregulated polyphenols and hormones. This study also identified a few valuable medicinal substances such as trigonelline and 5-hydroxytryptophan in T. asperellum TJ01 fermentation cultures.
Author wang, Gui S.
Wu, Qiong
Yu, Mei X.
Tang, Jun
Ni, Mi
Liu, Qian Q.
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  organization: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University
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Cites_doi 10.1038/srep40140
10.1002/jms.659
10.3390/molecules21060773
10.1002/rcm.3568
10.1111/j.1439-0434.2007.01330.x
10.1016/j.foodchem.2018.03.125
10.1128/AEM.01578-17
10.1007/s10725-018-0383-7
10.1016/j.soilbio.2007.02.015
10.1093/nar/gkn810
10.1093/pcp/pct176
10.1371/journal.pone.0179957
10.1007/978-981-10-0388-2_6
10.1016/j.cub.2010.02.042
10.1111/mpp.12429
10.1016/j.tibs.2017.01.004
10.1021/acs.analchem.7b04424
10.1515/hf-2013-0171
10.1093/nar/gkl923
10.1104/pp.108.130369
10.1104/pp.106.078428
10.1094/MPMI-09-13-0265-R
10.1146/annurev-phyto-073009-114314
10.1002/ffj.2730070412
10.1139/m85-144
10.1002/rcm.5188
10.1038/nprot.2011.335
10.1128/AEM.71.12.8587-8596.2005
10.1111/j.1432-1033.1990.tb19323.x
10.1007/978-1-4684-7112-0_12
10.1007/BF00396537
10.1002/jms.1777
10.3389/fmicb.2018.00612
10.1021/acs.jafc.6b00801
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Snippet Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to...
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SubjectTerms Alkaloids
Amino acids
Biological control
Culture
different time-points
Fermentation
Flavonoids
Fungi
Hormones
Lipid metabolism
Lipids
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Plant growth
Polyphenols
Quadrupoles
Secondary metabolites
Sugar
Trichoderma asperellum
triple-quadrupole mass spectrometry
Title Analysis of metabolic changes in Trichoderma asperellum TJ01 at different fermentation time-points by LC-QQQ-MS
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