Analysis of metabolic changes in Trichoderma asperellum TJ01 at different fermentation time-points by LC-QQQ-MS

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 54; no. 1; pp. 20 - 26
• Main Authors: Ni, Mi, Wu, Qiong, wang, Gui S., Liu, Qian Q., Yu, Mei X., Tang, Jun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.01.2019; Taylor & Francis Ltd
• Subjects: Alkaloids; Amino acids; Biological control; Culture; different time-points; Fermentation; Flavonoids; Fungi; Hormones; Lipid metabolism; Lipids; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Plant growth; Polyphenols; Quadrupoles; Secondary metabolites; Sugar; Trichoderma asperellum; triple-quadrupole mass spectrometry; different time-points; fermentation; metabolites; triple-quadrupole mass spectrometry
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2018.1507227

Trichoderma spp. are among the most widely recognized biocontrol fungi used to inhibit pathogens and promote plant growth. These functions are related to primary and secondary metabolites. This study investigated the different metabolites in Trichoderma asperellum TJ01 cultured for 24 and 72 h using liquid chromatography with triple-quadrupole mass spectrometry. Compared to the 24 h culture of T. asperellum TJ01, the 72 h culture with amino acid metabolism tended to decrease while sugar and lipid metabolisms tended to increase. Furthermore, the 72 h culture had a higher proportion of upregulated flavonoids, in combination with a higher proportion of downregulated alkaloids, and equal proportions of upregulated and downregulated polyphenols and hormones. This study also identified a few valuable medicinal substances such as trigonelline and 5-hydroxytryptophan in T. asperellum TJ01 fermentation cultures.