Bioremediation model for atrazine contaminated agricultural soils using phytoremediation (using Phaseolus vulgaris L.) and a locally adapted microbial consortium

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 52; no. 6; pp. 367 - 375
• Main Authors: Madariaga-Navarrete, Alfredo, Rodríguez-Pastrana, Blanca Rosa, Villagómez-Ibarra, José Roberto, Acevedo-Sandoval, Otilio Arturo, Perry, Gregory, Islas-Pelcastre, Margarita
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.06.2017; Taylor & Francis Ltd
• Subjects: Abuse; Agricultural land; Agricultural practices; Agriculture; Atrazine; Atrazine - analysis; Atrazine - metabolism; Atrazine degradation; Augmentation; Beans; Biodegradation, Environmental; Bioremediation; Conidia; Consortia; Flowers & plants; Greenhouses; Herbicides; Herbicides - analysis; Herbicides - metabolism; Inoculation; locally adapted microorganism consortium; Mexico; Microbial Consortia; Microorganisms; Models, Biological; Phaseolus - drug effects; Phaseolus - physiology; Phaseolus vulgaris; Phytoremediation; Rhizobium - drug effects; Rhizobium - metabolism; Rhizosphere; Soil contamination; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - metabolism; Soils; Trichoderma - drug effects; Trichoderma - metabolism; locally adapted microorganism consortium; bioremediation; phytoremediation; Atrazine degradation
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2017.1292092

The objective of the present study was to examine a biological model under greenhouse conditions for the bioremediation of atrazine contaminated soils. The model consisted in a combination of phytoremediation (using Phaseolus vulgaris L.) and rhizopheric bio-augmentation using native Trichoderma sp., and Rhizobium sp. microorganisms that showed no inhibitory growth at 10,000 mg L −1 of herbicide concentration. 33.3 mg of atrazine 50 g −1 of soil of initial concentration was used and an initial inoculation of 1 × 10 9 UFC mL −1 of Rhizobium sp. and 1 × 10 5 conidia mL −1 of Trichoderma sp. were set. Four treatments were arranged: Bean + Trichoderma sp. (B+T); Bean + Rhizobium sp. (BR); Bean + Rhizobium sp. + Trichoderma sp. (B+R+T) and Bean (B). 25.51 mg of atrazine 50 g −1 of soil (76.63%) was removed by the B+T treatment in 40 days (a = 0.050, Tukey). This last indicate that the proposed biological model and methodology developed is useful for atrazine contaminated bioremediation agricultural soils, which can contribute to reduce the effects of agrochemical abuse.