Genetic analysis of Anisakis typica (Nematoda: Anisakidae) from cetaceans of the northeast coast of Brazil: New data on its definitive hosts

• Published in: Veterinary parasitology Vol. 178; no. 3; pp. 293 - 299
• Main Authors: Iñiguez, Alena M., Carvalho, Vitor L., Motta, Monica R. Alves, Pinheiro, Diana C. Sousa Nunes, Vicente, Ana C. Paulo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.06.2011
• Subjects: Animals; Anisakiasis - parasitology; Anisakiasis - veterinary; Anisakid; Anisakidae; Anisakis - anatomy & histology; Anisakis - genetics; Anisakis - isolation & purification; Anisakis typica; Aspergillus; Base Sequence; Brazil; Brazilian coast; Breviceps; Cetacea; Cetacea - parasitology; Cetacean host; DNA, Helminth - chemistry; DNA, Helminth - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Electra; Genetic Variation - genetics; ITS region; Kogia breviceps; Marine; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction - veterinary; Polymorphism, Single Nucleotide - genetics; RNA, Ribosomal, 5.8S - chemistry; RNA, Ribosomal, 5.8S - genetics; Sequence Alignment; Stenella longirostris; Steno bredanensis; Brazil; ASW, Brazil, Ceara; Cetacean host; ITS region; Anisakid; Anisakis typica; Brazilian coast
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2011.01.001

Anisakids from 5 different species of cetacean, Kogia breviceps, Peponocephala electra, Stenella clymene, Stenella longirostris and Steno bredanensis, were submitted to genetic analysis. Adults and larvae fixed in ethanol–formalin–acetic acid or in 70% ethanol for periods ranging from 10 months to 10 years were isolated from 9 cetaceans stranded on Ceará coast, Northeast Brazil. The 18S rDNA gene, ITS1, and specific Anisakis typica ITS regions were amplified by PCR. 18S rDNA and ITS1 region confirmed Anisakis sp. morphological identification but also detected the presence of Aspergillus sp. in longer preserved samples. All samples were identified as A. typica by ITS species-specific PCR. The study report three new definitive hosts of A. typica from the Brazilian Atlantic coast by genetic analysis: P. electra, K. breviceps, and S. clymene.