Potent immunogenicity of an HIV-1 gag– pol fusion DNA vaccine delivered by in vivo electroporation
A plasmid DNA vaccine containing a fusion gene consisting of an HIV-1 subtype C gag and a modified subtype C pol was compared to a mixture of gag plus pol or gag plus HIV env plasmids. Plasmid DNA was delivered by intramuscular injection followed by electroporation in vivo. Two vaccinations were suf...
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Published in | Vaccine Vol. 24; no. 21; pp. 4503 - 4509 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Ltd
22.05.2006
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Subjects | |
Online Access | Get full text |
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Summary: | A plasmid DNA vaccine containing a fusion gene consisting of an HIV-1 subtype C
gag and a modified subtype C
pol was compared to a mixture of
gag plus
pol or
gag plus HIV
env plasmids. Plasmid DNA was delivered by intramuscular injection followed by electroporation in vivo. Two vaccinations were sufficient to induce high levels of Gag- and Pol-specific CD4 and CD8 T cells in peripheral blood. The
gag–
pol fusion plasmid was as immunogenic as the plasmid mixtures. Thus, DNA vaccination by intramuscular electroporation was an effective means for inducing high levels of Gag- and Pol-specific T cells, and a single
gag–
pol fusion DNA vaccine was sufficient for eliciting immune responses against both antigens. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0264-410X 1873-2518 |
DOI: | 10.1016/j.vaccine.2005.08.017 |