Characterization of mutations in HIV type 1 isolates from 144 Cambodian recently infected patients and pregnant women naive to antiretroviral drugs

A baseline study has been conducted to determine the polymorphism of reverse transcriptase, protease, and envelope genes of HIV-1 isolates from 146 antiretroviral drug-naive Cambodian patients including 22 seroconverters and 124 pregnant women having been diagnosed HIV positive for less than 1 year....

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Published inAIDS research and human retroviruses Vol. 21; no. 11; p. 971
Main Authors Ly, Nary, Recordon-Pinson, Patricia, Phoung, Viseth, Srey, Chanthan, Kruy, Leang Sim, Koum, Kanal, Chhum, Vannarith, Glaziou, Philippe, Fleury, Herve J, Reynes, Jean-Marc
Format Journal Article
LanguageEnglish
Published United States 01.11.2005
Subjects
Amino Acid Sequence
Amino Acid Substitution - genetics
Cambodia
Drug Resistance, Viral - genetics
Female
Genes, env
HIV Infections - virology
HIV Protease - genetics
HIV Reverse Transcriptase - genetics
HIV-1 - classification
HIV-1 - genetics
HIV-1 - isolation & purification
Humans
Molecular Sequence Data
Mutation
Polymorphism, Genetic
Pregnancy
Pregnancy Complications, Infectious - virology
Recombination, Genetic
Sequence Alignment
Sequence Analysis, DNA
Cambodia
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Summary:A baseline study has been conducted to determine the polymorphism of reverse transcriptase, protease, and envelope genes of HIV-1 isolates from 146 antiretroviral drug-naive Cambodian patients including 22 seroconverters and 124 pregnant women having been diagnosed HIV positive for less than 1 year. Amplification of at least one gene was successful for 144 isolates. All three genes were obtained for 136 isolates. Subtyping showed that CRF01_AE was predominant (130 cases). According to the ANRS September 2004 list, polymorphism substitutions (>50% versus the subtype B consensus) of CRF01_AE at drug resistance positions were observed only in protease: I13V (81%), E35D (87%), M36I (100%), R41K (96%), and H69K (100%). Two strains bore one major resistance mutation to PIs: M46I and N88D. Five other strains carried drug resistance mutations to RTIs: K70R (one strain), V75M (three strains), and K101E (one strain). Of the isolates 4.9% had drug resistance mutations to antiretroviral drugs.
ISSN:0889-2229
1931-8405
DOI:10.1089/aid.2005.21.971