Determination of polyphenolic compounds in wastewater olive oil by gas chromatography–mass spectrometry

• Published in: Talanta (Oxford) Vol. 70; no. 1; pp. 213 - 218
• Main Authors: Zafra, A., Juárez, M.J.B., Blanc, R., Navalón, A., González, J., Vílchez, J.L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.2006; Oxford Elsevier
• Subjects: Analysis methods; Analytical chemistry; Antioxidants; Applied sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Gas chromatographic methods; Gas chromatography–mass spectrometry (GC–MS); Global environmental pollution; Liquid–liquid microextraction (LLME); Natural water pollution; Pollution; Polyphenols; Silylation; Spectrometric and optical methods; Wastewater olive oil (WWOO); Water treatment and pollution; Antioxidants; Liquid–liquid microextraction (LLME); Polyphenols; Wastewater olive oil (WWOO); Gas chromatography–mass spectrometry (GC–MS); Silylation; Trace analysis; Chemical analysis; Internal standard; Gas chromatography-mass spectrometry (GC-MS); Microanalysis; Secondary ion mass spectrometry; Olive oil; Antioxidant; Waste water; Liquid-liquid microextraction (LLME); Gas chromatography; Polyphenol; Mass spectrometry MS/MS; Microextraction; Environment; Mass spectrometry; Monitoring; Quantitative analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2005.12.038

A simple and sensitive method for the determination of 21 polyphenolic compounds in wastewater from olive oil production plants is proposed. The method involves a liquid–liquid microextraction (LLME) procedure with ethyl acetate followed by a silylation step. Identification and quantification have been performed by gas chromatography–mass spectrometry (GC–MS). MS measurements were carried out using selected ion monitoring mode (SIM). α-Naftol was used as internal standard. The proposed method was applied to the determination of these compounds in wastewater from an olive oil production factory in Jaén (Spain) at concentration levels ranging from 1.0 to 75.0 μg ml −1 for each compound. The autodegradation process by own microbiota in samples collected in three different points of the factory was also studied. The method was validated by a recovery assay with spiked samples.