Effect of Stathmin Decoy-oligodeoxynucleotides on the Proliferation and Differentiation of Precartilainous Stem Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 5; pp. 557 - 560
• Main Author: 郭风劲 张衣北 陈安民
• Format: Journal Article
• Language: English
• Published: China Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 01.10.2007
• Subjects: Animals; Cartilage - cytology; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Oligodeoxyribonucleotides - genetics; Oligodeoxyribonucleotides - pharmacology; Rats; Rats, Sprague-Dawley; Stathmin - genetics; Stathmin - pharmacology; Stem Cells - cytology; 低聚脱氧核苷; 前软骨干细胞; 细胞凋亡; 细胞增殖; differentiation; Stathmin gene; proliferation; decoy-oligodeoxynucleotides; precartilainous stem cells
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0521-3

By using decoy-oligodeoxynucleotides （decoy-ODNS） technique, the effects of Stathmin gene on the proliferation and differentiation of in vitro cultured precartilainous stem cells （PSCs） were investigated. The Stathmin decoy-ODNs were transfected into PSCs in rats by using gene transfection technique. Under the induction of cortisol （1 μmol/L）, electrophoretic mobility shift assay was used the inhibitory effects of decoy-ODNS on Stathmin gene. MTT and cytometry were used to test the cell proliferation. The expression of collagen Ⅱ and Ⅴ and Stathmin protein was detected by using Western blot. The results showed that Stathmin decoy-ODNs inhibited the Stathmin activity in a dose-dependent manner. When the concentration of decoy-ODNs was 10 times of standard con- centration, the proliferation of PSCs was obviously suppressed and the differentiation happened. Compared to the control group, the difference was significant （P〈0.05）. It was concluded that decoy-ODNs could inhibit the proliferation and promote the differentiation of PSCs by antagonizing Stathmin activity.