Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations
A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonuclei...
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| Published in | Journal of chromatography Vol. 140; no. 3; p. 251 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
21.10.1977
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| Subjects | |
| Online Access | Get more information |
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| Abstract | A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3% or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used. |
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| AbstractList | A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3% or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used. |
| Author | Seibert, G Zahn, R K Breter, H J |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/914914$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Chromatography, High Pressure Liquid Chromatography, Ion Exchange Deoxyribonucleosides - isolation & purification DNA - analysis Drug Contamination Methods Ribonucleosides - isolation & purification RNA - analysis |
| Title | Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations |
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