Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations

A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonuclei...

Full description

Saved in:
Bibliographic Details
Published inJournal of chromatography Vol. 140; no. 3; p. 251
Main Authors Breter, H J, Seibert, G, Zahn, R K
Format Journal Article
LanguageEnglish
Published Netherlands 21.10.1977
Subjects
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Deoxyribonucleosides - isolation & purification
DNA - analysis
Drug Contamination
Methods
Ribonucleosides - isolation & purification
RNA - analysis
Online AccessGet more information

Cover

More Information
Summary:A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3% or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used.
DOI:10.1016/S0021-9673(00)93584-2