Production of cellulase complex by Trichoderma reesei rut-C30 on lignocellulose and its hydrolytic potential
Trichoderma reesei Rut-C30 grew well on glucose with apparent specific growth rates of 0·16 h −1 and 0·12 h −1, on Mandels' and Vogel's media, respectively. Similar specific growth rates (0·15 h −1 and 0·11 h −1) were recorded when grown on pure cellulose. This organism produced 0·30 IU/ml...
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Published in | Biomass Vol. 2; no. 2; pp. 127 - 137 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.01.1982
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Subjects | |
Online Access | Get full text |
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Summary: | Trichoderma reesei Rut-C30 grew well on glucose with apparent specific growth rates of 0·16 h
−1 and 0·12 h
−1, on Mandels' and Vogel's media, respectively. Similar specific growth rates (0·15 h
−1 and 0·11 h
−1) were recorded when grown on pure cellulose. This organism produced 0·30 IU/ml cellulase activity on 1% glucose. The activity increased to approximately 1 IU/ml when 1% cellulose was used as the sole carbon source. Cellulase activity further increased to 3·5 IU/ml and a productivity of 37·2 IU/litre/h with a pure cellulose concentration of 2·5%. A cellulase activity of 1·0 IU/ml was obtained on 1% cellulose from 56V, a lignocellulosic material produced by the Iotech process. When the concentration of 56V was increased to 2·5% cellulose, no growth was seen because of an increase in toxic and/or inhibitory compounds associated with an increase in substrate concentration. However, a high cellulase activity of 3·7 IU/ml and a productivity of 37·0 IU/litre/h was obtained on 56 V when the toxic and/or inhibitory compounds were removed by water washing. This proves that 56V is as good a substrate as pure cellulose for enzyme production. The enzyme produced with water-washed 56V gave about 89% of the theoretical yield of glucose on hydrolysis of whole or delignified 56V. |
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ISSN: | 0144-4565 1878-2523 |
DOI: | 10.1016/0144-4565(82)90022-1 |