Myoglobin entrapment in poly(vinyl alcohol) dense membranes

Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesi...

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Published inBrazilian journal of chemical engineering Vol. 31; no. 3; pp. 747 - 756
Main Authors Figueiredo, K. C. S., Alves, T. L. M., Borges, C. P.
Format Journal Article
LanguageEnglish
Portuguese
Published Brazilian Society of Chemical Engineering 01.09.2014
Subjects
ENGINEERING, CHEMICAL
Myoglobin
Immobilization
Membranes
Poly(vinyl alcohol)
Oxygen/nitrogen separation
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Summary:Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions in order to maintain the native protein folding. Membrane characterization comprised the water swelling degree, DSC, TGA, UV-visible spectroscopy, FTIR analysis and oxygen transport in a dialysis cell. The incorporation of myoglobin in the film decreased the water swelling degree and improved the membrane thermal properties compared to unmodified PVA membrane. The reduction of ferric iron in the prosthetic group of the protein to the ferrous form was observed. The increased affinity between oxygen and the immobilized myoglobin did not favor the release of this solute from the biocarrier.
ISSN:0104-6632
1678-4383
0104-6632
DOI:10.1590/0104-6632.20140313s00002307