Evaluation of CD7 and terminal deoxynucleotidyl transferase (TdT) expression in CD34+ myeloblasts from patients with myelodysplastic syndrome

• Published in: Leukemia research Vol. 30; no. 8; pp. 957 - 963
• Main Authors: Font, P., Subirá, D., Mtnez-Chamorro, C., Castañón, S., Arranz, E., Ramiro, S., Gil-Fernández, J.J., López-Pascual, J., Alonso, A., Pérez-Sáenz, M.A., Alaez, C., Renedo, M., Blas, C., Escudero, A., Fdez-Rañada, J.M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2006
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, CD34 - biosynthesis; Antigens, CD7 - analysis; Antigens, CD7 - biosynthesis; CD34 myeloblasts; CD7; Cytogenetic Analysis; DNA Nucleotidylexotransferase - analysis; DNA Nucleotidylexotransferase - biosynthesis; Female; Flow Cytometry; Follow-Up Studies; Granulocyte Precursor Cells - immunology; Humans; Immunophenotyping; Male; Middle Aged; Myelodysplastic Syndromes - diagnosis; Myelodysplastic Syndromes - immunology; Risk Factors; Survival Rate; TdT; Flow cytometry; Immunophenotyping; CD34 myeloblasts; TdT; CD7
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2005.11.026

There is an emerging use of flow cytometry to evaluate patients with myelodysplastic syndrome (MDS). We have studied CD7 and TdT expression in the CD34+ myeloid blast cell population in 55 bone marrow samples of patients with MDS. CD7 and/or TdT were detected in 38 out of 55 patients (69%). CD7 expression was not related to other bad prognosis data but conversely, we found an association between TdT+ CD34 myeloblasts and high-risk MDS patients according to the International Prognostic Scoring System. Therefore, CD7 and TdT may help to establish the diagnosis of MDS and, TdT expression also seems to be a useful marker in distinguishing risk groups.