Bimodular thrombin aptamers with two types of non-covalent locks

Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of...

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Published inNucleosides, nucleotides & nucleic acids Vol. 40; no. 5; pp. 559 - 577
Main Authors Alieva, Rugiya, Novikov, Roman, Tashlitsky, Vadim, Arutyunyan, Alexander, Kopylov, Alexey, Zavyalova, Elena
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 2021
Taylor & Francis Ltd
Subjects
Aptamer
Aptamers
DNA nanoconstruction
G-quadruplex
non-covalent lock
Oligonucleotides
Thrombin
non-covalent lock
Aptamer
thrombin
DNA nanoconstruction
G-quadruplex
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Summary:Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of self-assembling locks were used to create bimodular aptamer constructions. Well-known aptamer to thrombin was chosen as a model object. The assembly of duplex locks was more efficient at low concentrations. The functional activity of aptamer modules was nearly the same as in HD1. However, the affinity of bimodular aptamers with G-quadruplex locks to immobilized thrombin was 5-10 times higher.
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ISSN:1525-7770
1532-2335
1532-2335
DOI:10.1080/15257770.2021.1910297