In vitro anti-inflammatory activity of spherical silver nanoparticles and monodisperse hexagonal gold nanoparticles by fruit extract of Prunus serrulata: a green synthetic approach

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 46; no. 8; pp. 2022 - 2032
• Main Authors: Singh, Priyanka, Ahn, Sungeun, Kang, Jong-Pyo, Veronika, Soshnikova, Huo, Yue, Singh, Hina, Chokkaligam, Mohan, El-Agamy Farh, Mohamed, Aceituno, Verónica Castro, Kim, Yeon Ju, Yang, Deok-Chun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.12.2018; Taylor & Francis Ltd
• Subjects: Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Biocompatibility; Cell activation; Chemical compounds; Cyclooxygenase-2; Fruit - chemistry; Gene Expression Regulation - drug effects; Gold; Gold - chemistry; Gold - pharmacology; gold nanoparticles; Green synthesis; Inflammation; Lipopolysaccharides; Macrophages; MAP kinase; MAP Kinase Signaling System - drug effects; Metal Nanoparticles - chemistry; Mice; Nanoparticles; Nanospheres - chemistry; NF-κB; NF-κB protein; Nitric oxide; Nitric-oxide synthase; P. serrulata; Pharmacology; Plant Extracts - chemistry; Prostaglandin E2; Prunus - chemistry; RAW 264.7 Cells; Signal transduction; Silver; Silver - chemistry; Silver - pharmacology; silver nanoparticles; Synthesis; Visual observation; NF-κB; Green synthesis; P. serrulata; silver nanoparticles; gold nanoparticles; inflammation
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2017.1408117

Recently, green metal nanoparticles have received global attention owing to their economical synthesis, biocompatible nature, widespread biomedical and environmental applications. Current study demonstrates a sustainable approach for the green synthesis of silver nanoparticles (P-AgNPs) and gold nanoparticles (P-AuNPs) from P. serrulata fresh fruit extract. The silver and gold nanoparticles were synthesized in a very rapid, efficient and facile manner, within 50 min and 30 s at 80 °C, respectively. The nanoparticles were characterized by using visual observation, UV-Vis, FE-TEM, EDX, elemental mapping, FT-IR, XRD and DLS, which confirmed the formation of monodispersed, crystalline and stable nanoparticles. Further, we explored these nanoparticles for anti-inflammatory activity through inhibition of downstream NF-κB activation in macrophages (RAW264.7). We demonstrated that the nanoparticles reduced expression of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PEG 2 ), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was attenuated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Furthermore, nanoparticles significantly suppressed LPS-induced activation of NF-κB signalling pathway via p38 MAPK in RAW 264.7 cells. To the best of our knowledge, this is the first report on the efficient green synthesis of P-AgNPs and P-AuNPs using P. serrulata fresh fruit extract and its in vitro anti-inflammatory effects. Collectively, our results suggest that P. serrulata fresh fruit extract is a green resource for the eco-friendly synthesis of P-AgNPs and P-AuNPs, which further can be utilized as a novel therapeutic agent for prevention and cure of inflammation due to their biocompatible nature.