Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice

• Published in: Molecular biology reports Vol. 38; no. 4; pp. 2557 - 2562
• Main Authors: Liang, Min, Yang, Jian Ling, Bian, Min Juan, Liu, Jie, Hong, Xiao Qi, Wang, Yan Cong, Huang, Yu Fang, Gu, Shu Ping, Yu, Mei, Huang, Fang, Fei, Jian
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.04.2011; Springer Netherlands; Springer Nature B.V
• Subjects: Amino acids; Animal Anatomy; Animal Biochemistry; Animals; Base Sequence; Biomedical and Life Sciences; blood glucose; Cloning, Molecular; DNA Primers - genetics; Embryo, Mammalian - metabolism; embryogenesis; Embryonic Development - genetics; Embryonic Development - physiology; Endocrine System - metabolism; Gene Targeting - methods; Histology; Life Sciences; messenger RNA; Mice; Mice, Mutant Strains; Molecular biology; Molecular Sequence Data; Morphology; Nerve Tissue Proteins - metabolism; Polymerase chain reaction; Protein Isoforms - metabolism; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Rodents; Sequence Analysis, DNA; Embryonic development; RESP18-c; Gene targeting; Expression; RESP18
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-010-0394-6

Regulated endocrine-specific protein-18 (RESP18) is distributed mainly in the peripheral endocrine and neuroendocrine tissues. The expression of RESP18 protein is regulated by physiological factors, such as blood glucose or dopaminergic drugs, but its functions remain unclear. In this study, to explore the biological functions of RESP18 in vivo, we generated RESP18 heterozygous deficient mice, and further found RESP18 was essential for embryonic development. In addition, we cloned a new isoform of mouse RESP18 by reverse transcription-polymerase chain reaction (RT-PCR), and denominated it as RESP18-c. Mouse RESP18-c, by skipping exon4 (43 bp in length), encodes a shorter protein of 120 amino acid residues. The distribution of RESP18-c mRNA is similar with that of RESP18 mRNA in the peripheral tissues and brains of mice.