Adenylated proteins in mouse B16-F10 melanoma cells cluster in functional categories: a new paradigm for cellular regulation?

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 41; no. 3; pp. 255 - 263
• Main Authors: Fatima, Narjis, Alomari, Munther, Belov, Larissa, Shen, Yandong, Christopherson, Richard I.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 2022; Taylor & Francis Ltd
• Subjects: Adenosine; Adenosine Monophosphate - metabolism; AMP; AMPylated; Animals; Antibodies; Cytoskeleton; endoplasmic reticulum stress; GRP78; Huntingtin; hypoxia-inducible factor; Lysates; Mammals - metabolism; mass spectrometry; Mass spectroscopy; Melanoma; Mice; Phosphate esters; Post-translation; Protein folding; Protein Processing, Post-Translational; Proteins; Tyrosine; unfolded protein response; unfolded protein response; GRP78; mass spectrometry; melanoma; hypoxia-inducible factor; AMPylated; endoplasmic reticulum stress
• ISSN: 1525-7770; 1532-2335; 1532-2335
• DOI: 10.1080/15257770.2021.1995608

In mammals, AMPylation of cellular proteins is carried out by Huntingtin yeast-interacting protein E, and pseudokinase SelO. Lysates from mouse B16-F10 melanoma cells have been fractionated by immuno-precipitation using magnetic Dynabeads coated with antibodies against both adenosine 5′-monophosphate in phosphate ester linkage to tyrosine, and adenosine-phosphate. Proteins pulled down with both these antibodies were subject to post-translational modification, most likely AMPylation. Using tandem mass spectrometry, analysis of these protein fractions identified 333 proteins that could be pulled down by both antibodies. Many of these proteins clustered in 13 functional Ingenuity Pathway Analysis categories of 4 or more adenylated proteins including some from the cytoskeleton, and some involved with initiating the unfolded protein response. Supplemental data for this article is available online at https://doi.org/10.1080/15257770.2021.1995608 .