Myoblast-Acellular Skeletal Muscle Matrix Constructs Guarantee a Long-Term Repair of Experimental Full-Thickness Abdominal Wall Defects
To obtain a valuable treatment of congenital muscle defect, cell-matrix constructs composed of satellite cell-derived myoblasts (XY karyotype) seeded on muscle acellular matrices were used to repair a previously created full-thickness defect of abdominal wall of 18 1-month-old female Lewis rats. Ace...
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Published in | Tissue engineering Vol. 12; no. 7; pp. 1929 - 1936 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Mary Ann Liebert, Inc
01.07.2006
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Subjects | |
Online Access | Get full text |
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Summary: | To obtain a valuable treatment of congenital muscle defect, cell-matrix constructs composed of satellite
cell-derived myoblasts (XY karyotype) seeded on muscle acellular matrices were used to repair a previously
created full-thickness defect of abdominal wall of 18 1-month-old female Lewis rats. Acellular
abdominal matrices, obtained by a detergent-enzymatic method, were positive for both basic fibroblast
growth factor and transforming growth factor-β, and were able to support
in vitro
cell adhesion. All
animals survived the surgery, without signs of infection or implant rejection, and were humanely killed at 1,
3, or 9 months after surgery. The implants appeared well preserved, were integrated in the host tissue, and
maintained their original dimension and thickness until 9 months. Vesicular acetylcholine transporter was
expressed on the surface of muscle fibers from 1 month postsurgery. Finally, implanted male myoblasts
were present inside the patches until 9 months, as demonstrated by the expression of SrY mRNA and by the
presence of Y chromosome probe signal. These results allow us to conclude that cell-matrix constructs
could represent a promising approach to the repair of muscle defects, because they are repopulated
in vivo
by skeletal muscle cells and nervous elements and maintain their structural integrity over the long term. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1076-3279 1557-8690 |
DOI: | 10.1089/ten.2006.12.1929 |