Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS

4-Nonylphenol (NP) and octylphenol (OP) were measured by direct ELISA in both laboratory-fortified and surface water samples collected monthly from 10 rivers. In this procedure, samples were concentrated by solid phase extraction (SPE) using Lichrolut RP-18 sorbent with good recoveries obtained for...

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Published inTalanta (Oxford) Vol. 70; no. 4; pp. 745 - 751
Main Authors Céspedes, R., Skryjová, K., Raková, M, Zeravik, J., Fránek, M., Lacorte, S., Barceló, D.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 15.11.2006
Oxford Elsevier
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Summary:4-Nonylphenol (NP) and octylphenol (OP) were measured by direct ELISA in both laboratory-fortified and surface water samples collected monthly from 10 rivers. In this procedure, samples were concentrated by solid phase extraction (SPE) using Lichrolut RP-18 sorbent with good recoveries obtained for both LC–MS and ELISA, giving a low level of detection (LOD) at the range of low μg L −1 and good reproducibility. Analysis of 40 surface water samples demonstrated that the ELISA may be a useful screening tool for the determination of the alkylphenols in surface water matrices. The concentration of NP and OP in surface waters ranged from 0.11 to 6.58 μg L −1. A good correlation of results obtained by ELISA and LC–MS within the concentration range of 0.08–6.86 μg L −1 was found in the river samples [ R 2 = 0.924, n = 39]. The influence of various factors on assay determination was also discussed.
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content type line 23
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2006.07.001