Alternative splicing reprogramming in fungal pathogen Sclerotinia sclerotiorum at different infection stages on Brassica napus
Alternative splicing (AS) is an important post-transcriptional mechanism promoting the diversity of transcripts and proteins to regulate various life processes in eukaryotes. Sclerotinia stem rot is a major disease of Brassica napus caused by Sclerotinia sclerotiorum, which causes severe yield loss...
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Published in | Frontiers in plant science Vol. 13; p. 1008665 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
12.10.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Alternative splicing (AS) is an important post-transcriptional mechanism promoting the diversity of transcripts and proteins to regulate various life processes in eukaryotes. Sclerotinia stem rot is a major disease of
Brassica napus
caused by Sclerotinia sclerotiorum, which causes severe yield loss in
B. napus
production worldwide. Although many transcriptome studies have been carried out on the growth, development, and infection of S. sclerotiorum, the genome-wide AS events of S. sclerotiorum remain poorly understood, particularly at the infection stage. In this study, transcriptome sequencing was performed to systematically explore the genome-scale AS events of
S. sclerotiorum
at five important infection stages on a susceptible oilseed rape cultivar. A total of 130 genes were predicted to be involved in AS from the
S. sclerotiorum
genome, among which 98 genes were differentially expressed and may be responsible for AS reprogramming for its successful infection. In addition, 641 differential alternative splicing genes (DASGs) were identified during
S. sclerotiorum
infection, accounting for 5.76% of all annotated
S. sclerotiorum
genes, and 71 DASGs were commonly found at all the five infection stages. The most dominant AS type of
S. sclerotiorum
was found to be retained introns or alternative 3′ splice sites. Furthermore, the resultant AS isoforms of 21 DASGs became pseudogenes, and 60 DASGs encoded different putative proteins with different domains. More importantly, 16 DASGs of
S. sclerotiorum
were found to have signal peptides and possibly encode putative effectors to facilitate the infection of
S. sclerotiorum
. Finally, about 69.27% of DASGs were found to be non-differentially expressed genes, indicating that AS serves as another important way to regulate the infection of
S. sclerotiorum
on plants besides the gene expression level. Taken together, this study provides a genome-wide landscape for the AS of
S. sclerotiorum
during infection as well as an important resource for further elucidating the pathogenic mechanisms of
S. sclerotiorum
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Shuijin Hua, Zhejiang Academy of Agricultural Sciences, China; Zheng Wang, Jiangsu University, China; Yong Liu, Sichuan Academy of Agricultural Sciences, China These authors have contributed equally to this work This article was submitted to Plant Pathogen Interactions, a section of the journal Frontiers in Plant Science Edited by: Qi Peng, Jiangsu Academy of Agricultural Sciences (JAAS), China |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.1008665 |