A resveratrol oligomer, hopeaphenol suppresses virulence activity of Pectobacterium atrosepticum via the modulation of the master regulator, FlhDC
Pectobacterium atrosepticum ( P. atrosepticum : Pba ) which causes potato soft rot and blackleg is a notorious plant pathogen worldwide. Discovery of new types of antimicrobial chemicals that target specifically to virulence factors such as bacterial motility and extracellular enzymes is required fo...
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Published in | Frontiers in microbiology Vol. 13; p. 999522 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
28.10.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Pectobacterium atrosepticum
(
P. atrosepticum
:
Pba
) which causes potato soft rot and blackleg is a notorious plant pathogen worldwide. Discovery of new types of antimicrobial chemicals that target specifically to virulence factors such as bacterial motility and extracellular enzymes is required for protecting crops from pathogenic infection. A transcriptomic analysis of
Pba
upon hopeaphenol treatment revealed that bacterial motility-related gene expression, including a master regulator
flhDC
genes, was significantly influenced by hopeaphenol. We further generated a double knock-out mutant of
flhDC
genes by CRISPR/Cas9 system and confirmed phenotypic changes in bacterial motility, transcription of extracellular enzymes, and disease development consistent with the result of wild-type treated with hopeaphenol. The hopeaphenol-treated
Pba
strains, wild-type, double mutant, and complemented strain were unable to secrete the enzymes
in vitro
, while Δ
flhDC
double mutant strain reduced the secretion. Thus, our study supports that FlhDC is essential for the virulence of
Pba
, and proposes that hopeaphenol modulates FlhDC-dependent virulence pathways, suggesting a potential of hopeaphenol as an anti-virulence agent to manage potato soft rot and blackleg diseases. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Roshni R. Kharadi, Michigan State University, United States; Rita Monson, University of Cambridge, United Kingdom These authors have contributed equally to this work Edited by: Guoqing Niu, Southwest University, China This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.999522 |