Detection of pim-1 mRNA in prostate cancer diagnosis

• Published in: Chinese medical journal Vol. 120; no. 17; pp. 1491 - 1493
• Main Authors: He, Hui-chan, Bi, Xue-cheng, Dai, Qi-shan, Wang, Shao-sheng, Wei, Hong-ai, Zhong, Wei-de, Liu, Wen-hua, Jiang, Fu-neng, Liu, Liang-shi
• Format: Journal Article
• Language: English
• Published: China Department of Urology, First People's Hospital of Guangzhou,Guangzhou Medical College, Guangzhou 510180, China%College of Life Science, Sun Yat-sen University, Guangzhou 510180, China 05.09.2007
• Subjects: Aged; Humans; Male; Middle Aged; Polymerase Chain Reaction; Prostate - metabolism; Prostatic Hyperplasia - metabolism; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - metabolism; Proto-Oncogene Proteins c-pim-1 - genetics; RNA, Messenger - analysis; Sensitivity and Specificity; prostate neoplasms; proto-oncogene proteins c-pim-1; diagnosis
• ISSN: 0366-6999; 2542-5641
• DOI: 10.1097/00029330-200709010-00005

Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa. The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve. The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively). Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.