Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine

• Published in: Talanta (Oxford) Vol. 88; pp. 129 - 135
• Main Authors: Lee, Tien Ping, Saad, Bahruddin, Khayoon, Wejdan Shakir, Salleh, Baharuddin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2012; Elsevier
• Subjects: Adsorption; Adult; Analytical chemistry; Applied sciences; Beverages - analysis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Coffee; Coffee - chemistry; Exact sciences and technology; Food Contamination; Global environmental pollution; High performance liquid chromatography; Humans; Micro-solid phase extraction; Middle Aged; Molecular Imprinting; Molecularly imprinted polymer; Mycotoxins - urine; Ochratoxin A; Ochratoxins - urine; Other chromatographic methods; Pollution; Porosity; Sensitivity and Specificity; Solid Phase Microextraction - methods; Sonication; Vitis - chemistry; Micro-solid phase extraction; Coffee; Ochratoxin A; Molecularly imprinted polymer; High performance liquid chromatography; Urine; Solid phase extraction; Sonication; Molecular structure; Porous membrane; Sample; HPLC chromatography; Polymer; Method; Chemical enrichment; Sorption; Sample preparation; Detection limit; Environment; Technique; Molecular imprinting; Ultrasound
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.10.021

► Molecularly imprinted polymer (MIP) as sorbent in micro-solid phase extraction for the determination of ochratoxin A (OTA). ► The method exploits the high specificity of the MIP and the simplicity of the μ-SPE. ► The method offers comparable sensitivity with the LC–MS/MS methods. ► The developed method can be an interesting alternative sample preparation over the commonly used IAC procedure. A simple, environmental friendly and selective sample preparation technique employing porous membrane protected micro-solid phase extraction (μ-SPE) loaded with molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) is described. After the extraction, the analyte was desorbed using ultrasonication and was analyzed using high performance liquid chromatography. Under the optimized conditions, the detection limits of OTA for coffee, grape juice and urine were 0.06ngg−1, 0.02 and 0.02ngmL−1, respectively while the quantification limits were 0.19ngg−1, 0.06 and 0.08ngmL−1, respectively. The recoveries of OTA from coffee spiked at 1, 25 and 50ngg−1, grape juice and urine samples at 1, 25 and 50ngmL−1 ranged from 90.6 to 101.5%. The proposed method was applied to thirty-eight samples of coffee, grape juice and urine and the presence of OTA was found in eighteen samples. The levels found, however, were all below the legal limits.