Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

• Published in: Chemical speciation and bioavailability Vol. 26; no. 2; pp. 111 - 118
• Main Authors: Ali, Daoud, Gend Kumar, Phool, Kumar, Sudhir, Ahmed, Mukhtar
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 2014
• Subjects: Channa punctata; Channa punctatus; cholinesterase; comet assay; dimethoate; DNA damage; enzyme inhibitors; exposure duration; freshwater fish; genotoxicity; glutathione; lethal concentration 50; lipid peroxidation; mutagens; oxidative stress; stress response; superoxide dismutase
• ISSN: 0954-2299; 2047-6523
• DOI: 10.1080/09542299.2014.11073965

Dimethoate is one of the organophosphate insecticides widely used in agriculture throughout the world and is an inhibitor of cholinesterase in animals. The objective of the present study was to detect oxidative stress and DNA damage induced by dimethoate in the freshwater fish Channa punctatus (C. punctatus). The LC 50 -96 h value of technical grade dimethoate was estimated at 19.10 μg L -1 in a semi-static system and, on the basis of the LC 50 value, three concentrations were determined. The fish were exposed to these concentrations of dimethoate for 96 h and samplings were done at 24 and 96 h for assessment of oxidative stress and DNA damage. After exposure to dimethoate, the level of superoxide dismutase declined while lipid peroxidation, glutathione, induction of micronucleus and DNA damage were increased in C. punctatus as the concentration and exposure time increased. Thus our results suggest that dimethoate induces genotoxic effects which invariably accompanied and correlated with oxidative stress.