Sequence Homologies and Linkage Group Conservation of the Human and Mouse Cenpc Genes

• Published in: Genomics (San Diego, Calif.) Vol. 22; no. 1; pp. 36 - 40
• Main Authors: McKay, Stewart, Thomson, Eric, Cooke, Howard
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.1994; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Centromere - metabolism; Chromosomal Proteins, Non-Histone - genetics; Chromosome Mapping; Chromosomes, Human, Pair 4; Cloning, Molecular; Conserved Sequence; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Linkage; Humans; In Situ Hybridization; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Pseudogenes; Sequence Homology, Amino Acid; Species Specificity; Genetic mapping; Human; Pseudogene; Nucleotide sequence; Rodentia; Chromosome 5; Chromosome 2; B4-Chromosome; Vertebrata; Mammalia; Complementary DNA; Gene; Mouse; C12-Chromosome; Linkage group
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1994.1342

Using a previously identified human CENPC cDNA fragment, we have isolated cDNA clones corresponding to the complete mouse Cenpc coding sequence. Using these cDNAs as probes to genomic libraries, we have isolated genomic clones corresponding to the mouse and human genes and also to a mouse pseudogene. In situ hybridization mapping of these genes reveals that the human gene maps to 4q12-q13.3 and the mouse gene to 5E2-E5. These sites are in a region of linkage group conservation between the two species. Secondary sites are present in man on chromosome 12q21.2-q21.33 and in mouse on chromosome 2B. This mouse secondary site is a pseudogene on the basis of DNA sequence. These secondary sites are not syntenic in the two species.