Isolation and genomic characterization of Klebsiella Lw3 with polychlorinated biphenyl degradability

• Published in: Environmental technology Vol. 44; no. 24; pp. 3656 - 3666
• Main Authors: Zhu, Di-Hua, Nie, Fang-Hong, Song, Qing-Lang, Wei, Wan, Zhang, Min, Hu, Yao, Lin, Hong-Ying, Kang, Dan-Ju, Chen, Zhi-Bao, Chen, Jin-Jun
• Format: Journal Article
• Language: English; French
• Published: England Taylor & Francis 28.10.2023; Taylor & Francis Ltd
• Subjects: 16S rRNA; Bacteria; Biodegradability; Biodegradation; Bioremediation; Carbon sources; Contamination; Degradability; Degradation; Gas chromatography; Genetic structure; Genomic analysis; Genomic sequencing; Gram-negative bacteria; Inorganic salts; Klebsiella; Mangrove; Mass spectrometry; Mass spectroscopy; Organic compounds; PCB; Pesticide residues; Pesticides; Phylogeny; Polychlorinated biphenyl; Polychlorinated biphenyls; rRNA 16S; Sediments; Klebsiella; 16S rRNA; Degradability; Genomic sequencing; Mangrove; Polychlorinated biphenyl
• ISSN: 0959-3330; 1479-487X
• DOI: 10.1080/09593330.2022.2068381

Bioremediation of sediment organic pollution has been intensely investigated, but the degradation of complex organic compounds, pesticide residues, and polychlorinated biphenyls (PCBs) remains poorly studied. In this study, sediments were collected from Zhanjiang Mangrove Reserve and inoculated in an inorganic salt medium using only biphenyl (BP) and PCBs as the carbon sources to obtain a PCB-degrading strain. A gram-negative bacterium that metabolized PCBs was isolated and identified as Klebsiella Lw3 by 16S rDNA phylogenetic analysis. Genomic sequencing showed that this bacterium possessed genes related to BP/PCB degradation, and its GC content was 58.2%; we identified 3326 cellular pathways. Gas chromatography-mass spectrometry was employed to test the PCB degrading ability; the results showed that the strain had a good degradation effect on PCB3 at concentrations of 5, 10, 20, 40, and 60 mg/L and that the final degradation rate was higher than 97% after 96 h. Interestingly, this strain showed good biodegradability of PCBs despite having no classical PCB degradation pathway, providing a new direction for Klebsiella research with practical significance for in situ bioremediation of PCB contamination. Overall, this study provides valuable insights into the genetic structure of PCB-degrading strains as well as eco-friendly and low-cost PCB degradation and lays a foundation for the discovery of new degradation pathways.