Delivery of alpha-tocopherol through soluble dietary fibre-based nanofibres for improving the life span of Caenorhabditis elegans

• Published in: International journal of food sciences and nutrition Vol. 70; no. 2; pp. 172 - 181
• Main Authors: Li, Juan, Chotiko, Arranee, Chouljenko, Alexander, Gao, Chenfei, Zheng, Jolene, Sathivel, Subramaniam
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 17.02.2019; Taylor & Francis Ltd
• Subjects: Aging; alpha-tocopherol; alpha-Tocopherol - administration & dosage; alpha-Tocopherol - pharmacology; Animals; C. elegans; Caenorhabditis elegans; Caenorhabditis elegans - drug effects; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - genetics; Dietary Fiber; Food science; Heat shock; Heat-Shock Response; Life span; Longevity - drug effects; Mutants; Mutation; Nanofibers; nanofibre; Nematodes; Oxidative Stress; pharyngeal pumping rate; Pharynx; Pumping; soluble dietary fibre; Succinate Dehydrogenase - genetics; Tocopherol; Worms; nanofibre; pharyngeal pumping rate; soluble dietary fibre; life span; alpha-tocopherol
• ISSN: 0963-7486; 1465-3478
• DOI: 10.1080/09637486.2018.1489785

The effect of alpha-tocopherol (α-TOC) delivered by soluble dietary fibre-based nanofibres (α-TOC-SDNF) on the life span of nematode Caenorhabditis elegans N2 (wild type) and TK22 (mev-1 mutants) with and without heat shock was investigated. Without heat shock, the wild-type and mev-1 mutants maintained in the 100 µg/mL of α-TOC-SDNF had longer life spans than their respective blank control groups. With heat shock, the wild-type N2 in the 200 µg/mL of α-TOC-SDNF had a survival rate of 5% at day 49, while no nematodes survived in the blank control group. An increased pharyngeal pumping rate was observed in the α-TOC-SDNF treated mev-1 mutants worms compared to the blank control group. Encapsulating α-TOC in SDNF yielded protective effects and the life span and pumping rate of C. elegans was increased with α-TOC delivered by SDNF.