Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (Approved Lists 1980)

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 11; pp. 2589 - 2596
• Main Authors: Vandecandelaere, Ilse, Nercessian, Olivier, Segaert, Eveline, Achouak, Wafa, Mollica, Alfonso, Faimali, Marco, De Vos, Paul, Vandamme, Peter
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2008; Society for General Microbiology
• Subjects: Alteromonas - classification; Alteromonas - genetics; Alteromonas - isolation & purification; Alteromonas - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biofilms - growth & development; Biological and medical sciences; DNA Fingerprinting; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Electrodes - microbiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genotype; Italy; Microbiology; Miscellaneous; Molecular Sequence Data; Phenotype; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Species Specificity; Stainless Steel; Systematics; Italy; Marine environment; Description; Taxonomy; Biofilm; Bacteria; New species; Alteromonas
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65691-0

1 Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium 2 LEMiR, Laboratoire d'Ecologie Microbienne de la Rhizosphère, CNRS-CEA-Université de la Méditerranée, DEVM, Dpt Ecophysiologie Végétale et de Microbiologie/DSV CEA Cadarache, 13108 Saint Paul Lez Durance, France 3 Instituto di Scienze Marine – Consiglio Nazionale delle Ricerche (ISMAR-CNR), Via de Marini 6, 16149 Genoa, Italy Correspondence Ilse Vandecandelaere Ilse.Vandecandelaere{at}UGent.be Thirty-five isolates obtained from a marine electroactive biofilm grown on a stainless steel cathode (Genoa, Italy) were investigated by a polyphasic taxonomic approach. Whole-cell fatty acid methyl ester analysis indicated that the isolates belonged to the class Gammaproteobacteria and were closely related or belonging to the genus Alteromonas . Genomic fingerprinting using the BOX-PCR primer delineated five clusters of isolates with similar BOX-PCR fingerprints. This study demonstrated that isolates from four of the BOX-PCR clusters belonged to Alteromonas macleodii and that the 14 isolates representing BOX-PCR cluster 1 constituted a novel species, which shared 98.4 % 16S rRNA gene sequence similarity with its nearest phylogenetic neighbour, Alteromonas hispanica . Both phenotypic and genotypic analyses enabled this novel species, for which the name Alteromonas genovensis sp. nov. is proposed, to be differentiated from established species of the genus Alteromonas . The DNA G+C content of Alteromonas genovensis sp. nov. is 44.5 mol% and the type strain is LMG 24078 T (=CCUG 55340 T ). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LMG 24078 T , LMG 24079, LMG 24080, LMG 24081, LMG 24082 and LMG 24083 are AM885866 , AM887686 , AM885868 , AM885867 , AM885870 and AM887685 , respectively. DNA–DNA hybridization results and additional electron micrographs are available with the online version of this paper.