Polyether ionophores residues in Minas Frescal cheese by UHPLC-MS/MS

• Published in: Food additives & contaminants Part B, Surveillance communications Vol. 13; no. 2; pp. 130 - 138
• Main Authors: Silva, Felipe R. N., Bortolotte, Andrea R., Braga, Patricia A. de C., Reyes, Felix G. R., Arisseto-Bragotto, Adriana P.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.06.2020; Taylor & Francis Ltd
• Subjects: antibiotics; anticoccidials; Cheese; Coefficient of variation; food safety; Linearity; Mathematical analysis; Monensin; polyether ionophores; Residues; Veterinary drug residues; food safety; polyether ionophores; anticoccidials; Veterinary drug residues; antibiotics; cheese
• ISSN: 1939-3210; 1939-3229
• DOI: 10.1080/19393210.2020.1739149

An analytical method was developed and validated for the determination of three polyether ionophores (monensin, lasalocid, and salinomycin) in 60 samples of Brazilian Minas Frescal cheese by UHPLC-MS/MS. Linearity ranged from 1 to 8 μg kg −1 for monensin and salinomycin, and from 0.50 to 4 μg kg −1 for lasalocid. Limits of detection and quantitation were 0.50 μg kg −1 and 1 μg kg −1 , respectively, for both monensin and salinomycin, and 0.25 μg kg −1 and 0.50 μg kg −1 , respectively, for lasalocid. Recoveries were between 69% and 84% with coefficients of variation up to 16.28% for repeatability and 13.79% for intermediate precision. A total of 60 samples of Minas Frescal cheese were analysed and only monensin residues were found. Monensin was detected in 55% of the samples and quantified in 5 of them at mean levels varying from 1.00 to 1.73 μg kg −1 . The proposed method demonstrated the suitability for monitoring these substances in cheese.