Analysis of ruminant respiratory syncytial virus isolates by RNAse protection of the G glycoprotein transcripts

Two different respiratory syncytial virus (RSV) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant RSV isolates by the ribonuclease protection assay. A 32P-radiolabeled antisense RNA probe was transcribed from cloned ovine and bovine RSV G glycoprotein genes and t...

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Bibliographic Details
Published inJournal of veterinary diagnostic investigation Vol. 11; no. 3; pp. 215 - 220
Main Authors Alansari, H, Duncan, R.B, Baker, J.C, Potgieter, L.N.D
Format Journal Article
LanguageEnglish
Published United States 01.05.1999
Subjects
Animals
Bovine respiratory syncytial virus
Cattle
Cattle Diseases - genetics
Cattle Diseases - virology
Genetic Variation
glycoproteins
Glycoproteins - genetics
Goat Diseases - genetics
Goat Diseases - virology
Goats
heterogeneity
Phosphorus Radioisotopes
Pneumovirus
Respiratory Syncytial Viruses - genetics
ribonucleases
RNA - genetics
RNA probes
Sheep
Sheep Diseases - genetics
Sheep Diseases - virology
Viral Envelope Proteins - genetics
United States
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Summary:Two different respiratory syncytial virus (RSV) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant RSV isolates by the ribonuclease protection assay. A 32P-radiolabeled antisense RNA probe was transcribed from cloned ovine and bovine RSV G glycoprotein genes and then hybridized with total RNA isolated from infected cells with various ruminant RSV isolates. The results of this study, along with previously published nucleotide sequence data of the ovine RSV G glycoprotein gene, suggest the presence of at least 2 ruminant RSV subgroups. One subgroup is represented by RSV isolated from respiratory disease outbreaks from calves and goats, and the other is represented by RSV isolated from sheep.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063879901100302