New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring
Lately, the use of zebrafish has gained increased interest in the scientific community as an animal model in preclinical research. However, there is a lack of in vivo imaging tools that ensure animal welfare during acquisition procedures. The use of functional imaging techniques, like Positron Emiss...
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Published in | Frontiers in physiology Vol. 13; p. 906110 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
29.06.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Lately, the use of zebrafish has gained increased interest in the scientific community as an animal model in preclinical research. However, there is a lack of
in vivo
imaging tools that ensure animal welfare during acquisition procedures. The use of functional imaging techniques, like Positron Emission Tomography (PET), in zebrafish is limited since it requires the animal to be alive, representing a higher instrumentation complexity when compared to morphological imaging systems. In the present work, a new zebrafish enclosure was developed to acquire
in vivo
images while monitoring the animal’s welfare through its heartbeat. The temperature, dissolved oxygen, and pH range in a closed aquatic environment were tested to ensure that the conditions stay suitable for animal welfare during image acquisitions. The developed system, based on an enclosure with a bed and heartbeat sensors, was tested under controlled conditions in anesthetized fishes. Since the anesthetized zebrafish do not affect the water quality over time, there is no need to incorporate water circulation for the expected time of PET exams (about 30 min). The range of values obtained for the zebrafish heart rate was 88–127 bpm. The developed system has shown promising results regarding the zebrafish’s heart rate while keeping the fish still during the long imaging exams. The zebrafish enclosure ensures the animal’s well-being during the acquisition of
in vivo
images in different modalities (PET, Computer Tomography, Magnetic Resonance Imaging), contributing substantially to the preclinical research. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Aquatic Physiology, a section of the journal Frontiers in Physiology William Holmes, University of Glasgow, United Kingdom Reviewed by: Yonghe Ding, Mayo Clinic, United States Edited by: Jose Fernando Lopez-Olmeda, University of Murcia, Spain |
ISSN: | 1664-042X 1664-042X |
DOI: | 10.3389/fphys.2022.906110 |