Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

• Published in: Genetic resources and crop evolution Vol. 54; no. 4; pp. 865 - 874
• Main Authors: Liu, Yong, Xiong, Zhi-Yong, He, Yong-Gang, Shewry, Peter R, He, Guang-yuan
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Kluwer Academic Publishers 01.06.2007; Springer Nature B.V
• Subjects: Alleles; Bread; Composition; Electrophoresis; Gel electrophoresis; Genetic diversity; genetic variation; Glutenin; HMW glutenin subunit; Molecular weight; Population genetics; Population levels; Populations; Sodium dodecyl sulfate; Sodium lauryl sulfate; Subunit structure; Triticum aestivum; Triticum aestivum L; Wheat; Wheat landraces
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-006-9154-9

The high molecular weight (HMW) glutenin subunit composition of 111 common landraces of bread wheat collected from Hubei province, China has been determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Ninety six of the accessions were homogeneous for HMW glutenin subunit composition and 15 were heterogeneous. For the Glu-1 loci, 16 alleles were detected, 3 at the Glu-A1locus, 9 at the Glu-B1and 4 at the Glu-D1. Three novel alleles were identified, two at the Glu-B1 and one at the Glu-D1locus. Combination of these 16 alleles resulted in 14 different HMW subunit patterns. The distribution of HMW glutenin subunit alleles in a subset of 105 of the 111 accessions representing six populations was assessed both at the individual population and whole population levels. The results demonstrated that the distribution of allelic patterns varied among populations. Taken together, 62.5% of the alleles detected were considered to be rare alleles while the Glu-A1c (null), Glu-B1b (1Bx7 + 1By8) and Glu-D1a (1Dx2 + 1Dy12) alleles were found most frequently in the six populations. The subset exhibited relatively high genetic diversity (A = 5.33, P = 1.00, Ae = 1.352 and He = 0.238) with 81.5% of the diversity being within populations and 18.5% between populations.