Kocuria gwangalliensis sp. nov., an actinobacterium isolated from seawater

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 11; pp. 2769 - 2772
• Main Authors: Seo, Yong Bae, Kim, Dong-Eun, Kim, Gun-Do, Kim, Hyun-Woo, Nam, Soo-Wan, Kim, Young Tae, Lee, Jae Hyung
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2009; Society for General Microbiology
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Systematics; Actinomycetales; Kocuria; Taxonomy; Bacteria; Actinomycetes; New species; Seawater
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.008482-0

1 Department of Microbiology, Pukyong National University, Busan 608-737, Republic of Korea 2 Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea 3 Department of Marine Biology, Pukyong National University, Busan 608-737, Republic of Korea 4 Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Republic of Korea 5 Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Republic of Korea 6 Basic Science Research Institute, Pukyong National University, Busan 608-737, Republic of Korea Correspondence Jae Hyung Lee lenio{at}pknu.ac.kr A novel aerobic, non-motile, Gram-positive, pink–orange-pigmented, coccoid marine bacterium, designated strain SJ2 T , was isolated from the Gwangalli coast of Korea. The taxonomic position of strain SJ2 T was determined based on 16S rRNA gene sequence analysis, fatty acid patterns and physiological reaction profiles. The full-length 16S rRNA gene sequence of strain SJ2 T showed highest similarity to those of the type strains of Kocuria carniphila and Kocuria marina . Strain SJ2 T exhibited mean levels of DNA–DNA relatedness of 17 and 35 % to the type strains of K. carniphila and K. marina , respectively. Based on these results, strain SJ2 T is considered to represent a novel species of the genus Kocuria , for which the name Kocuria gwangalliensis sp. nov. is proposed. The type strain is SJ2 T (=KCCM 42914 T =LMG 24672 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SJ2 T is EU286964 . A transmission electron micrograph of cells of strain SJ2 T and maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic position of strain SJ2 T among members of the genus Kocuria are available as supplementary material with the online version of this paper.