Batch and fed-batch fermentation of optically pure D (-) lactic acid from Kodo millet (Paspalum scrobiculatum) bran residue hydrolysate: growth and inhibition kinetic modeling

A low-cost Kodo millet bran residue was utilized as feedstock for the production of D (−) lactic acid (DLA) using Lactobacillus delbrueckii NBRC3202 under anaerobic condition. Data culled from a series of batch fermentation processes with different initial Kodo millet bran residue hydrolysate (KMBRH...

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Published inPreparative biochemistry & biotechnology Vol. 50; no. 4; pp. 365 - 378
Main Authors Balakrishnan, Rengesh, Tadi, Subbi Rami Reddy, Rajaram, Shyam Kumar, Mohan, Naresh, Sivaprakasam, Senthilkumar
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 20.04.2020
Taylor & Francis Ltd
Subjects
Accuracy
Anaerobic conditions
Anaerobic processes
Batch culture
Computer simulation
D (−) lactic acid
Fermentation
Growth rate
kinetic modeling
Lactic acid
Luedeking-Piret equation
Millet
millet bran
Process intensification
Product inhibition
Residues
Substrate inhibition
D (−) lactic acid
product inhibition
Luedeking-Piret equation
millet bran
substrate inhibition
kinetic modeling
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Summary:A low-cost Kodo millet bran residue was utilized as feedstock for the production of D (−) lactic acid (DLA) using Lactobacillus delbrueckii NBRC3202 under anaerobic condition. Data culled from a series of batch fermentation processes with different initial Kodo millet bran residue hydrolysate (KMBRH) and DLA concentrations were used for kinetic model development. Both simulated and experimental data were in good agreement for cell growth, KMBRH utilization, and DLA formation. The values of kinetic constants specific growth rate, (μ m = 0.17 h −1 ); growth (α P = 0.96 g.g −1 ) and non-growth (β P = 1.19 g.g −1 .h −1 ) associated constant for DLA production and the maximum specific KMBRH utilization rate, (q G, max = 1.18 g.g −1 .h −1 ) were in good agreement with the literature reports. Kinetic analysis elucidated that L. delbrueckii growth was predominantly influenced by KMBRH limitation and highly sensitive to DLA inhibition. Fed-batch fermentation studies demonstrated the existence of substrate and product inhibition paving the scope for process intensification.
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ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2019.1697934