Genetic characterization of donkeys of Braj region of India

• Published in: Animal biotechnology Vol. 33; no. 7; pp. 1588 - 1590
• Main Authors: Behl, Rahul, Niranjan, S. K., Behl, Jyotsna, Arora, Reena, Singh, P. K., Vijh, R. K.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 12.12.2022; Taylor & Francis Ltd
• Subjects: Alleles; Animals; Braj region; Diversity; Donkey; Equidae - genetics; Gene Frequency; Genetic diversity; Genetic Variation - genetics; Genetics, Population; Heterozygosity; Heterozygote; India; Microsatellite Repeats - genetics; Microsatellites; India; Microsatellites; Donkey; Diversity; Braj region
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2021.1919131

The grey type donkeys of Braj region of Uttar Pradesh in India were genetically characterized using ten heterologous microsatellite loci. At these loci the PCR product size ranged from 75-95 bp at locus HTG6 to 251-277 bp at locus COR18. The observed number of alleles varied from 4 (HTG15) to 10 (HTG7 and AHT4) with a mean of 7.50 ± 1.96. The observed heterozygosity ranged from 0.46 (HTG6 and NVHEQ54) to 0.79 (COR18) with a mean of 0.66 ± 0.12. The mean genetic diversity estimate (FIS) was 0.157. When these donkeys were compared on the basis of allelic frequency data at these loci to the brown type donkeys of Ladakh, Spiti and Rayalseema regions in the union territories/states of Ladakh, Himachal Pradesh and Andhra Pradesh, respectively, they clustered independently from these three donkey populations in a dendrogram based on Goldstein's average square distances indicating their genetic distinctness.