Rapid Method for the Simultaneous Determination of Flavonol Aglycones in Food Using u-HPLC Coupled with Heating Block Acidic Hydrolysis

• Published in: Journal of AOAC International Vol. 96; no. 5; pp. 1059 - 1064
• Main Authors: SHIM, You-Shin, SEUNGHEE KIM, DONGWON SEO, ITO, Masahito, NAKAGAWA, Hiroaki, PARK, Hyun-Jin, JAEHO HA
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01.09.2013; Oxford University Press
• Subjects: Analysis; Bioflavonoids; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Detectors; Flavones; Flavonoids; Flavonoids - analysis; Flavonols - analysis; Food Analysis - methods; Fundamental and applied biological sciences. Psychology; Heating; Hydrolysis; Kaempferols - analysis; Limit of Detection; Methods; Quercetin - analogs & derivatives; Quercetin - analysis; Time Factors; Hydrolysis; HPLC chromatography; Foodstuff; Method; Aglycone; Simultaneous measurement
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.12-439

A rapid method for the simultaneous determination of flavonol aglycones in food using ultra-high-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 micro m id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.