Partitioning of Hydrophobic Molecules to Liposome Membranes Can Induce Variations in their Micro-Polarity and Micro-Viscosity

• Published in: Solvent Extraction Research and Development, Japan Vol. 22; no. 1; pp. 79 - 85
• Main Authors: IWASAKI, Fumihiko, SUGA, Keishi, KONDO, Dai, UMAKOSHI, Hiroshi
• Format: Journal Article
• Language: English
• Published: Japan Association of Solvent Extraction 2015
• ISSN: 1341-7215; 2188-4765
• DOI: 10.15261/serdj.22.79

The micro-polarity and micro-viscosity of liposome membranes were evaluated to develop a platform for the localization of hydrophobic substrates in aqueous solution. The distribution ratios of benzaldoxime (BO) onto the zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposome and onto the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) liposome were almost the same, while those of benzonitrile oxide (BNO) onto DOTAP liposomes were higher than those of BO. Through the analysis of a polarity-sensitive 6-lauroyl-2-dimethylaminonaphthalene, the membrane surface of the DOTAP liposome was found to be dehydrated in the presence of substrates. Using a fluorescent probe 1,6-diphenyl-1,3,5-hexatriene, we found that the micro-viscosity of the DOTAP liposome membrane increased with BNO. These results indicate that the interaction of hydrophobic substrates induce variations in the microscopic membrane environment.