Water Potential Associated with Cell Elongation and Cell Division of Tissue-Cultured Carnation Plants

This work was undertaken to determine the growth parameters of Lockhart's equation for finding which component was predominantly contributing to the cell expansion rates of tissue-cultured carnation plants (Dianthus caryophyllus L.). The water potential of the culture media ranged from -0.02 to...

Full description

Saved in:
Bibliographic Details
Published inPlant Biotechnology Vol. 16; no. 2; pp. 115 - 121
Main Authors IKEDA, Takashi, NONAMI, Hiroshi, FUKUYAMA, Toshio, HASHIMOTO, Yasushi
Format Journal Article
LanguageEnglish
Published Tokyo Japanese Society for Plant Cell and Molecular Biology 1999
Japan Science and Technology Agency
Online AccessGet full text

Cover

Loading…
More Information
Summary:This work was undertaken to determine the growth parameters of Lockhart's equation for finding which component was predominantly contributing to the cell expansion rates of tissue-cultured carnation plants (Dianthus caryophyllus L.). The water potential of the culture media ranged from -0.02 to -0.51MPa so that water stress conditions could be applied. Cell expansion could be inhibited completely by adding 2, 4-dichlorophenoxyacetic acid (2, 4-D) and benzylaminopurine (BA) to the culture media to form callus tissue. The sizes of the water potential gradient between the water source and the elongating cells correlated to the speed of growth rates under nutrient deficiency and growth retardation induced by the plant hormones, indicating that cell expansion rates were mainly associated with how much water could be absorbed by the elongating cells regardless of changes in growth under osmotic stress and growth retardation induced by addition of 2, 4-D and BA.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.16.115