Monoclonal antibodies to rabbit haemorrhagic disease virus and their use in the diagnosis of infection

• Published in: Journal of general virology Vol. 71; no. 11; pp. 2593 - 2598
• Main Authors: Rodak, L, Granatova, M, Valicek, L, Smid, B, Vesely, T, Nevorankova, Z
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.1990; Society for General Microbiology
• Subjects: Animal viral diseases; Animals; Antibodies, Monoclonal - immunology; Biological and medical sciences; Blotting, Western; Capsid - analysis; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Hybridomas - immunology; Infectious diseases; Medical sciences; Rabbits - microbiology; Viral diseases; Virus Diseases - diagnosis; Virus Diseases - immunology; Virus Diseases - veterinary; Rodentia; Hybridoma; Rabbit; Monoclonal antibody; Lagomorpha; Veterinary medicine; Immunoperoxidase technique; Virus; Vertebrata; Calicivirus; Specificity; Mammalia; Mouse; Caliciviridae; Cross reaction; Immunoblotting assay; Diagnosis; Immunofluorescence; ELISA assay
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-71-11-2593

Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czechoslovakia Hybridomas producing monoclonal antibodies (MAbs) to rabbit haemorrhagic disease virus (RHDV) were prepared. Using Western blot (WB) analysis, the MAbs obtained were divided into two groups, one reacting with the major structural proteins of M r 61K and 38K, and the other giving negative reactions. Both groups of MAbs, however, reacted specifically with RHDV in ELISA and by immunoperoxidase (IP) and immunofluorescence (IF) tests with infected cells. As demonstrated by WB using RHDV-specific MAbs and a MAb to feline calicivirus (FCV) strain F9, the major structural (capsid) proteins of RHDV and FCV have very similar sizes ( M r 61K and 38K compared to 62K to 64K and 40K respectively). No cross-reactions of MAbs with proteins of the other virus were observed in WB analysis, ELISA, IP tests or IF. The high specificity and sensitivity of RHDV-specific MAbs make them suitable for the routine IP and IF diagnosis of RHDV in liver cells of rabbits dying after natural or experimental infections. Received 21 May 1990; accepted 20 July 1990.