bFGF and Poly‐RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation

• Published in: Advanced materials interfaces Vol. 5; no. 7
• Main Authors: Jiang, Nan, Wang, Yong, Yin, Yi‐Xia, Wei, Rui‐Peng, Ying, Guo‐Liang, Li, Bin‐Bin, Qiu, Tong, Rijn, Patrick, Tian, Ge, Yan, Qiong‐Jiao, Dai, Hong‐Lian, Busscher, Henk J., Li, Shi‐Pu, Yetisen, Ali K., Yang, Xiao‐Yu
• Format: Journal Article
• Language: English
• Published: Weinheim John Wiley & Sons, Inc 09.04.2018
• Subjects: Adhesive bonding; Aspartic acid; biointerface; Biomedical materials; Cell adhesion; Cell adhesion & migration; cell proliferation; cooperative effect; Differentiation; Gene expression; Glycine; growth factors; Hydrogen bonding; Hydrogen storage; Interfaces; Lactic acid; Nanofabrication; Regeneration; Stability; stem cell differentiation; Stem cells; Substrates
• ISSN: 2196-7350; 2196-7350
• DOI: 10.1002/admi.201700702

Biointerface design is widely used to functionalize biomaterials with controllable physicochemical properties. Functionalized biointerface provides a versatile platform to connect biological entities and nonbiogenic materials. Existing nanofabrication approaches to create such a nanostructured biointerface involve in low stability of the functionalized nanolayer and simple functionalities that limit its applicability. Here, a stable nanolayered synthetic polypeptide (poly[LA‐co‐(Glc‐alt‐Lys)] and modified with arginine‐glycine‐aspartic acid, PRGD)/basic fibroblast growth factor (bFGF) biointerface is created via structural matching, charge interaction, and hydrogen bonding. The cooperative effect of the PRGD/bFGF biointerface shows multiple functionalities in promoting stem cell adhesion by 33% increase in cell adhesion to poly(d,l‐lactic acid) substrate as compared to experiments on bare substrate as a control. Moreover, the biointerface enhances proliferation by 40% in cell density, potential differentiation by 62%, and gene expression by 40 and 80% respectively as compared to the control samples. The fabricated biointerface may have applications in nerve regeneration, tissue repair, and stem cell‐based therapy. A cooperative effect is developed by fabricating a stable nanolayered synthetic polypeptide (poly[LA‐co‐(Glc‐alt‐Lys)] and modified with arginine‐glycine‐aspartic acid)/basic fibroblast growth factor biointerface via structural matching and hydrogen bonding. Such a functionality‐stable biointerface shows multiple functionalities in promoting stem cell adhesion, proliferation in cell density, and differentiation. This biointerface may have applications in nerve regeneration, tissue repair, and cell therapy.