Interaction of agitoxin2, charybdotoxin, and iberiotoxin with potassium channels: Selectivity between voltage-gated and Maxi-K channels

• Published in: Proteins, structure, function, and bioinformatics Vol. 52; no. 2; pp. 146 - 154
• Main Authors: Gao, Ying-Duo, Garcia, Maria L.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2003
• Subjects: AgTX2; Amino Acid Sequence; Animals; binding; Binding Sites; Charybdotoxin - chemistry; Charybdotoxin - metabolism; ChTX; Hydrogen Bonding; IbTX; Kv1.3 Potassium Channel; Large-Conductance Calcium-Activated Potassium Channels; Maxi-K channel; Models, Molecular; molecular modeling; Molecular Sequence Data; mutagenesis; peptides; Peptides - chemistry; Peptides - metabolism; Peptides - pharmacology; Potassium Channel Blockers - chemistry; Potassium Channel Blockers - metabolism; Potassium Channels - chemistry; Potassium Channels - metabolism; Potassium Channels, Calcium-Activated - chemistry; Potassium Channels, Calcium-Activated - metabolism; Potassium Channels, Voltage-Gated - chemistry; Potassium Channels, Voltage-Gated - metabolism; Protein Binding; Protein Subunits; Scorpion Venoms - chemistry; Scorpion Venoms - metabolism; Sequence Alignment; Shaker channels; Toxins, Biological - chemistry
• ISSN: 0887-3585; 1097-0134
• DOI: 10.1002/prot.10341

To gain insight into the molecular determinants that define the specificity of interaction of pore‐blocking peptides, such as agitoxin 2 (AgTX2), charybdotoxin (ChTX), and iberiotoxin (IbTX) with the Shaker‐type voltage‐gated potassium channel Kv1.3, or the large‐conductance Ca2+‐activated K+ (Maxi‐K) channel, homology models of these channels were generated based on the crystal structure of the bacterial, KcsA, potassium channel. Peptide–channel complexes were analyzed to evaluate the predicted interaction interfaces between the peptides and the channels' outer vestibules. The docking model, for either AgTX2 or ChTX with the Kv1.3 channel, predicts a novel hydrogen bonding interaction between the Asn30 side‐chain of the peptide and the Asp381 side‐chain of the channel. This interaction is consistent with the >500‐fold decreased potency of both AgTX2 and ChTX mutants at position 30 for the Shaker channel [(Ranganathan et al., Neuron 1996;16:131–139); (Goldstein et al., Neuron 1994;12:1377–1388)]. This hydrogen bonding interaction also suggests that Gly30 in IbTX may be the critical determinant for its lack of activity against Shaker Kv channels. The model of the Maxi‐K channel reveals a narrower and more structurally restrained outer vestibule in which the aromatic residues Phe266 and Tyr294 may stabilize binding of IbTX and ChTX by π–π stacking with the aromatic residues Trp14 and Tyr36 of the peptides. This study also suggests that the extra net negative charge of IbTX is not related to the selectivity of this peptide for the Maxi‐K channel. Proteins 2003;52:146–154. © 2003 Wiley‐Liss, Inc.