Ultrasensitive determination of intracellular superoxide in individual HepG2 cells by microfluidic chip electrophoresis
A microchip electrophoresis method was established for the determination of intracellular superoxide (O 2 −) in individual HepG2 cells. Dihydroethidium (DHE) was used as the specific fluorescent probe to react with intracellular O 2 − to form the fluorescent 2-hydroxyethidium. Excellent resolution b...
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Published in | Talanta (Oxford) Vol. 75; no. 5; pp. 1227 - 1233 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
15.06.2008
Oxford Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | A microchip electrophoresis method was established for the determination of intracellular superoxide (O
2
−) in individual HepG2 cells. Dihydroethidium (DHE) was used as the specific fluorescent probe to react with intracellular O
2
− to form the fluorescent 2-hydroxyethidium. Excellent resolution between 2-hydroxyethidium and ethidium cation (E
+) can be achieved within 20
s. E
+ was reported to be generated from photochemical oxidation of DHE and interfere the determination of O
2
− with fluorescence microscopic technique. An extremely low detection limit of 2.0
amol was achieved owing to the minute sample volume and insignificant dispersion effect during microfluidic chip-based electrophoretic separation. Furthermore, only 2-hydroxyethidium peak was detected with the suggested single-cell analysis method, which indicates the photooxidation of DHE to E
+ could be blocked by isolating either oxygen or light from them. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/j.talanta.2008.01.017 |