Control of canine gastric emptying of fat by lipolytic products

• Published in: The American journal of physiology Vol. 266; no. 6; p. G1017
• Main Authors: Meyer, J.H, Elashoff, J.D, Domeck, M, Levy, A, Jehn, D, Hlinka, M, Lake, R, Graham, L.S, Gu, Y.G
• Format: Journal Article
• Language: English
• Published: United States 01.06.1994
• Subjects: ACEITES; Animals; CHIEN; COLLOIDE; COLOIDES; CORPS GRAS; DIETA; Dietary Fats, Unsaturated - metabolism; DIGESTION; Dogs; Dose-Response Relationship, Drug; Emulsions; ESTOMAC; ESTOMAGO; Fats - metabolism; Food; Gastric Emptying - physiology; GRASAS; HUILE; Lipase - metabolism; LIPOLISIS; LIPOLYSE; Lipolysis; PANCREAS; Pancreatin - pharmacology; PERRO; REGIME ALIMENTAIRE; Stomach - metabolism; Time Factors; Tissue Distribution; TRASTORNOS DIGESTIVOS; TRIACILGLICEROL LIPASA; TRIACYLGLYCEROL LIPASE; TROUBLE DIGESTIF; Water
• ISSN: 0002-9513; 2163-5773
• DOI: 10.1152/ajpgi.1994.266.6.g1017

Dietary fat is ingested in three forms: 1) in solid food, 2) as aqueous emulsions, and 3) as unemulsified, liquid oil. On the basis of a scant previous literature, we postulated that liquid fat (emulsions or oils) would empty from the stomach at speeds that varied with the amounts ingested but that this dynamic would be modulated by feedback inhibition from lipolytic products. To test these ideas, we used a gamma camera to track gastric emptying of (123)I-labeled fat in dogs with chronic pancreatic fistulas by which lipase was excluded from or replenished in the duodenum in varied amounts after dogs were fed 15-, 30-, and 60-g loads of liquid fat given with solid foods or as emulsions. We also tracked concurrent gastric emptying of 113m(In), which marked the solid food phase or the water phase of emulsions. In some studies, we used a potent and specific inhibitor (orlistat) of pancreatic and gastric lipases to assess how lipolytic products modulated emptying of liquid fat. In the absence of pancreatic enzymes, both oils and emulsions emptied initially at high speeds that varied with fat loads, but emptying slowed 20 min after ingestion of emulsions and 60 min after ingestion of unemulsified oil. Studies with orlistat indicated that these changes in rates resulted from liberation of gastric lipolytic products. Emptying of oil emulsions was not altered by duodenal replenishment with pancreatic enzymes, but emptying of unemulsified oil was inhibited in a dose-related fashion, such that maximal inhibition was achieved when pancreatic enzymes were replenished at greater than or equal to 40% of normal amounts. Studies with orlistat confirm that this dose-dependent slowing was due specifically to lipase Emptying of solid food was much more sensitive to replenishment with enzymes, so that a 10% replenishment maximally inhibited solid emptying