Bioinformatics Analysis of Lactoferrin Gene for Several Species

• Published in: Biochemical genetics Vol. 46; no. 5-6; pp. 312 - 322
• Main Authors: Kang, Jing-Fen, Li, Xiang-Long, Zhou, Rong-Yan, Li, Lan-Hui, Feng, Fu-Jun, Guo, Xiu-Li
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.06.2008; Springer US; Springer Nature B.V
• Subjects: Amino Acid Substitution; Amino acids; Animals; Biochemistry; Bioinformatics; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cattle; Computational Biology; Dogs; Genetic diversity; Genetic Variation; Human Genetics; Humans; Lactoferrin - genetics; Medical Microbiology; Mice; Oxidizing agents; Phylogeny; Polymorphism, Genetic; Rats; Species diversity; Zoology; Variation; gene; Species
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-008-9147-9

Much attention has been focused on the study of lactoferrin due to its function in antibacterial, antiviral, antifungal, anti-inflammatory, anti-oxidant, and immunomodulatory activities. A total of 60 lactoferrin (LF) gene sequences with the complete coding regions (CDS) and corresponding amino acids belonging to 11 species were analyzed, and the differentiation within and among the species was also studied. The results showed that most of the species have the stop codon TAA, with the variation of TGA for Mus musculus. The length of the LF gene with the complete CDS varies greatly, from 2,055 to 2,190 bp, due to deletion, insertion, and stop codon mutation resulting in elongation. Observed genetic diversity was higher among species than within species, and Sus scrofa had more polymorphisms than any other species. Novel amino acid variation sites were detected within several species (8 in Homo sapiens, 6 in Mus musculus, 6 in Capra hircus, 10 in Bos taurus, and 20 in Sus scrofa), which might be used to illustrate the functional variation. Differentiation of the LF gene was obvious among species, and the clustering result was consistent with the taxonomy in the National Center for Biotechnology Information.