High-level expression and characterization of Aspergillus niger ATCC 1015 xylanase B in Komagataella phaffii

• Published in: Applied biological chemistry Vol. 61; no. 4; pp. 373 - 381
• Main Authors: Liu, Taiyu, Zhang, Jianguo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2018; Springer Nature B.V; 한국응용생명화학회
• Subjects: Applied Microbiology; Aspergillus niger; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Cultivation; Cupric ions; Feed industry; Food; Food industry; Food safety; Komagataella phaffii; Optimization; pH effects; Temperature; Xylanase; 농학; Xylanase; Gene expression; Food safety
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1007/s13765-018-0368-2

Owing to the safety issues in food and feed industry, the GH11 xylanase B gene from Aspergillus niger ATCC 1015 was cloned and expressed in Komagataella phaffii . The highest xylanase B activity of 1827.19 U/ml was obtained after optimization of temperature, pH, and methanol addition through flask cultivation. The optimal temperature and pH were 55 °C and 5.0, respectively, and the highest relative activity of xylanase B reached 133.20% with the addition of 10 mmol/l cupric ions. Thus, the high-level recombinant xylanase B obtained in this study could have potential applications in food and feed industry.