Transcription factor AP2 controls cnidarian germ cell induction

Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that tra...

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Published inScience (American Association for the Advancement of Science) Vol. 367; no. 6479; pp. 757 - 762
Main Authors DuBuc, Timothy Q, Schnitzler, Christine E, Chrysostomou, Eleni, McMahon, Emma T, Febrimarsa, Gahan, James M, Buggie, Tara, Gornik, Sebastian G, Hanley, Shirley, Barreira, Sofia N, Gonzalez, Paul, Baxevanis, Andreas D, Frank, Uri
Format Journal Article
LanguageEnglish
Published United States 14.02.2020
Subjects
Adult Stem Cells - cytology
Adult Stem Cells - metabolism
Animals
Female
Gametogenesis - genetics
Gametogenesis - physiology
Gene Expression Regulation, Developmental
Germ Cells - cytology
Gonads - cytology
Gonads - embryology
Hydrozoa - cytology
Hydrozoa - embryology
Hydrozoa - genetics
Male
Transcription Factor AP-2 - genetics
Transcription Factor AP-2 - physiology
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Summary:Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in mutants. Forced expression of in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line-sequestering and germ line-nonsequestering animals.
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Current address: Biology Department, Swarthmore College, Swarthmore, PA 19081, USA
Current address: Centre for Organismal Studies (COS), Heidelberg University, Heidelberg 69120, Germany
This manuscript has been accepted for publication in Science. This version has not undergone final editing. Please refer to the complete version of record at http://www.sciencemag.org/. The manuscript may not be reproduced or used in any manner that does not fall within the fair use provisions of the Copyright Act without the prior, written permission of AAAS.
Current address: Sars Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgt 55, 5008 Bergen, Norway
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aay6782