formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices

1. Bilirubin conjugation in rat liver slices was reassessed by using analysis of ethyl anthranilate azopigments to estimate separately the formation of bilirubin mono- and di-glucuronides. 2. Conjugation in slices resembles the situation in vivo more closely than does microsomal conjugation, in that...

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Bibliographic Details
Published inBiochemical journal Vol. 179; no. 3; pp. 473 - 477
Main Authors Campbell, M.T, Dutton, G.J
Format Journal Article
LanguageEnglish
Published England 01.06.1979
Subjects
animal science
Animals
Bilirubin - analogs & derivatives
Bilirubin - biosynthesis
Bilirubin - metabolism
Glucuronates - biosynthesis
Glucuronates - metabolism
Hydrolysis
In Vitro Techniques
Liver - metabolism
livestock
Male
Microsomes, Liver - metabolism
Rats
zoology
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Summary:1. Bilirubin conjugation in rat liver slices was reassessed by using analysis of ethyl anthranilate azopigments to estimate separately the formation of bilirubin mono- and di-glucuronides. 2. Conjugation in slices resembles the situation in vivo more closely than does microsomal conjugation, in that diglucuronide is formed in appreciable quantity. 3. Both bilirubin mono- and di-glucuronides were present in slices in approximately equal amounts, but the monoglucuronide was the major product found in the incubation medium. 4. These results are discussed in relation to recent theories on the relationship between bilirubin mono- and di-glucuronide formation in vivo.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0306-3275
0264-6021
1470-8728
DOI:10.1042/bj1790473