A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy

Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels...

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Bibliographic Details
Published inJournal of immunological methods Vol. 79; no. 1; p. 99
Main Authors Contractor, S F, Holmes-Ievers, E, Morgan, A, Oakey, M, Staines, N A
Format Journal Article
LanguageEnglish
Published Netherlands 10.05.1985
Subjects
Alkaline Phosphatase - analysis
Alkaline Phosphatase - blood
Alkaline Phosphatase - metabolism
Antibodies, Monoclonal
Ascitic Fluid
Female
Fluorescent Antibody Technique
Humans
Immunoenzyme Techniques
Isoenzymes - metabolism
Nitrophenols - metabolism
Organophosphorus Compounds - metabolism
Placenta - enzymology
Pregnancy
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Summary:Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.
ISSN:0022-1759
DOI:10.1016/0022-1759(85)90396-5